Company Name | Number of Employees | Industry Sector |
| Munroe Regional Medical Center | 2,481 | Healthcare |
| Wal-Mart (combined) | 2,370 | Retail Sales |
| Ocala Regional Medical Center & West Marion Community Hospital | 1,725 | Healthcare |
| Publix Supermarkets (combined) | 1,275 | Retail Sales |
| AT&T | 1,000 | Support Services |
| Lockheed Martin | 810 | Manufacturing |
| Swift Transportation Company | 524 | Transportation |
| Cheney Brothers, Inc. | 507 | Distribution |
| The Centers | 483 | Healthcare |
| ClosetMaid | 460 | Manufacturing |
| Hospice of Marion County, Inc. | 452 | Healthcare |
| Childhood Development Services, Inc. | 371 | Education |
| On Top of The World Communities, Inc. | 348 | Real Estate Developer |
| Signature Brands, LLC | 303 | Manufacturing |
| Custom Window Systems, Inc | 302 | Manufacturing/Distribution |
| Kmart Corporation | 300 | Distribution |
| Townley Manufacturing Company, Inc. | 256 | Manufacturing |
| Elster AMCO Water, Inc. | 188 | Manufacturing |
| Cone Distributing, Inc. | 187 | Distribution |