Company Name | Number of Employees | Industry Sector |
| Munroe Regional Medical Center | 2,500 | Healthcare |
| Wal-Mart (combined) | 2,370 | Retail Sales |
| Publix Supermarkets (combined) | 1,404 | Retail Sales |
| Ocala Regional Medical Center & West Marion Community Hospital | 1,301 | Healthcare |
| AT&T | 1,000 | Support Services |
| E-ONE, Inc. | 850 | Manufacturing |
| Lockheed Martin | 790 | Manufacturing |
| ClosetMaid | 600 | Manufacturing |
| Cheney Brothers, Inc. | 507 | Distribution |
| The Centers | 440 | Healthcare |
| Swift Transportation Company | 435 | Transportation |
| Hospice of Marion County, Inc. | 418 | Healthcare |
| Signature Brands, LLC | 380 | Manufacturing |
| Kmart Corporation | 350 | Distribution |
| Custom Window Systems, Inc | 254 | Manufacturing/Distribution |
| Townley Manufacturing Company, Inc. | 205 | Manufacturing |
| SPX Dehydration and Air Filtration | 202 | Manufacturing |
| On Top of The World Communities, Inc. | 200 | Real Estate Developer |
| Elster AMCO Water, Inc. | 188 | Manufacturing |
| Cone Distributing, Inc. | 180 | Distribution |